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HIF-1α protein levels in response to hypoxia induction. (A,B) HIF-1α levels were measured using immunocytochemistry and western blot after exposure to hypoxia for the indicated periods. Results are mean ± SD of three independent experiments performed in duplicate. **P<0.01 vs. normoxia group; ##P<0.01 vs. 12 h hypoxia group. HIF-1α, hypoxia-inducible factor 1α.
