Figure 4.

M. leprae 3β-HSD is a source of reductive power. (A, B) NAD+ and NADP + reduction to NADH and NADPH, respectively, by 3β-HSD activity was measured in a temporal kinetic curve every 30 s for 20 min at 340 nm. (C) Cytochrome C reduction was determined measuring the reduced form of Cytochrome C at 550 nm every 30 s for 20 min. In all conditions M. leprae WCL was incubated with 200 µM cholesterol, alone (blue) or in the presence of 100 µM compound 1 (red). A condition without cholesterol addition was also included as a control of basal levels (black). Representative of 3 independent experiments.