FIG. 2.

Overexpression of wild-type AKT2 sensitizes NIH 3T3 cells to FTI-277-induced apoptosis. A TUNEL assay was used to detect FTI-277-induced apoptosis in AKT2- or H-ras-transformed NIH 3T3 cells in a medium containing 5% FCS (a to c) or 0.1% FCS (d to f). After 48 h of FTI-277 treatment, apoptosis was detected in AKT2-transformed (c) but not H-ras-transformed (b) and pcDNA₃-transfected (a) NIH 3T3 cells in 5% FCS medium. In 0.1% FCS culture medium, FTI-277-induced apoptosis was more prominent in AKT2 (f)- than H-ras (e)-transformed NIH 3T3 cells.