FIG. 6.
Activation of AKT2 following attachment to fibronectin: inhibition by FTI-277. OVCAR-3 cells were serum starved, treated with or without FTI-277 or LY294002, and replated on fibronectin- or polylysine-coated plates. AKT2 was immunoprecipitated with an anti-AKT2 monoclonal antibody, and the immunoprecipitates were subjected to an in vitro kinase assay using histone H2B as the substrate. The autoradiogram (top) and quantitation by phosphorimaging (bottom) show that AKT2 is activated by cell adhesion to fibronectin (lane 2) but not to polylysine (lane 1). Integrin-mediated AKT2 activation is inhibited by LY294002 (lane 3) and FTI-277 (lane 4).