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. 2021 Sep 17;297(4):101206. doi: 10.1016/j.jbc.2021.101206

Figure 2.

Figure 2

Purification and activity of mATGL288-Str expressed in Lemo21 (DE3) cells.A, schematic drawing of the mATGL288-Str variant. B, SDS-PAGE (12%) after affinity purification of mATGL288-Str. Lane 1, Pierce unstained protein MW marker. Lane 2, elution fraction after the StrepII tag purification. Lane 3, elution fraction from size-exclusion chromatography. Red arrow indicates mATGL288-Str (45.3 kDa), # and ## indicate DnaK (70.0 kDa) and GroEL (60.0 kDa), respectively. C, triacylglycerol hydrolytic activity of purified mATGL288-Str in basal and mCGI-58-stimulated (indicated by an asterisk) condition. Str, StrepII tag.