Table 2.
Primers used for this work
| Variant | Forward primer | Reverse primer | Vector |
|---|---|---|---|
| M1-D288 (mouse ATGL truncation) | AAGGAGATATACATATGGGATCCATGTCGGACTCAGAAGTCAATC | CCGCCACCACCAATCTGTTCTCTGTGAGC | pST50 |
| M1-D288 (mouse ATGL truncation) | TTTTCCCAGTCGAGCTCAATAATTTTGTTTAACTTTAAGAAGGAGA TATAC | TGTTATCCGCTGGTACCTGTACATTAGTGGTGGTGGG | pST44 |
| N-terminal His insertion | CACCATCACTCGGACTCAGAAGTCAATC | ATGGTGATGCATGGATCCCATATG TATATC | pST44 |
| F17A of mATGL288-Str | AGGCTGTGGCGCGCTGGGCGTTT | GCAAAACTGATGTTCCATTTGG | pST44 |
| S47A of mATGL288-Str | TTATGGTGCGGCGGCAGGCGCCC | ATGTGCGTAGCGTTGGCCAC | pST44 |
| SMT3 | GCCGGCAGCGCTTGGAGC | GCCCTGAAAATACAGGTTTTCTCCGCC | pST44 |