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. 2021 Sep 15;13(9):10908–10921.

Figure 3.

Figure 3

BA promoted ECM synthesis and adjusted the expressions of ECM regulating genes in alginate-chondrocyte beads. (A) The chondrocytes from the 3D alginate beads were stained by alcian blue staining. (B) Quantitative analysis of alcian blue staining in alginate-chondrocyte beads. (C, D) Immunohistochemical staining (C) and quantitative analysis (D) of SOX9 in chondrocytes from the 3D alginate beads with various treatments for 24 hours. (E-G) The relative expressions of SOX9 (E), ACAN (F) and Col2α (G) in chondrocytes from 3D alginate beads with various treatments for 24 hours by qRT-PCR. *P<0.05 vs control group; #P<0.05 vs IL-1β group; ΔP<0.05 vs IL-1β + BA group, n=3.