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. 2021 Sep 15;13(9):10218–10232.

Figure 5.

Figure 5

Overexpression of CDC45 increased MCM7 expression, hindered AML cell proliferation, and blocked the G1/S phase transition. A. The protein expression levels of CDC45 and MCM7 in pDONR223-CDC45-transfected HL-60 and THP-1 cells. B. The protein expression levels of CDC45 and MCM7 in mEmerald-MCM7-transfected HL-60 and THP-1 cells. C. Representative images from the EdU assay using HL-60 and THP-1 cells after transfection with vector 1 and WT-CDC45. D. Representative images from the EdU assay using HL-60 and THP-1 cells after transfection with vector 2 and WT-MCM7. The ratio of EdU-positive cells was calculated as (EdU-positive cells/Hoechst-stained cells) ×100%. E. Representative images from the cell cycle assay using HL-60 and THP-1 cells after transfection with vector 1 and WT-CDC45. F. Representative images from the cell cycle assay using HL-60 and THP-1 cells after transfection with vector 2 and WT-MCM7. The percentages of cells in each cell cycle phase were expressed using a histogram. V1 and vector 1: pDONR223-vector; C and WT-CDC45: pDONR223-CDC45; V2 and vector 2: mEmerald-vector; M and WT-MCM7: mEmerald-MCM7; *P<0.05.