Table 7.
Characteristics of epithelial ovarian cancer cell metabolism in relation to chemosensitive and chemoresistant cell lines
| Study Model | Major findings | Interpretation | References | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
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| Glycolytic-related findings | OXPHOS-related findings | |||||||||
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| Glycolytic enzymes | Lactate | Basal ECAR | Others | OXPHOS enzymes | Basal OCR | ROS | Others | |||
| -Chemoresistant human serous ovarian carcinoma (C200, PEO4) | ↔ LDHa | ↑ | ↑ GLUT1 | ↑↑ CoxVb | ↑ | ↑ | ↑ OCR/ECAR | -Chemoresistant cells had higher metabolic states than chemosensitive cells. | [51] | |
| -Chemosensitive human ovarian carcinoma (A2780, PEO1) (control) | ↑ glycolytic capacity | ↑ ATP | ||||||||
| ↑ respiratory reserve | ||||||||||
| ↑↑ PGC-1α | ||||||||||
| -Human platinum-resistant HGSC (PEA2) | ↓ PFK | ↓ | ↓ glycolytic capacity | ↔ | ↑ | ↑ maximal OCR | -Platinum resistant cells had higher OXPHOS but had lower glycolysis than platinum-sensitive cells. | [36] | ||
| -Human platinum-sensitive HGSC (PEA1) (control) | ↓ GAPDH | ↑ BEC index | ||||||||
| -Cisplatin resistant human ovarian carcinoma (SKOV3/DDP) | ↑ PFK | ↓ | ↓ | ↑ Glut1 | ↔ PDK1 | ↑ | ↑ | ↑ ATP | -Cisplatin resistant cells had higher OXPHOS and glucose metabolism than cisplatin-sensitive cells. | [52] |
| -Cisplatin sensitive human ovarian carcinoma (SKOV3) (control) | ↑ LDHA | ↑ glucose uptake | ||||||||
| -Chemoresistant human ovarian carcinoma (C13, HeyA8MDR) | ↑ PFK | ↑ | ↑ glucose uptake | ↓ | ↑ ATP | -Chemoresistant cells had higher glycolysis compared to sensitive cells. | [19] | |||
| -Chemosensitive human ovarian carcinoma (HeyA8, OV2008) (control) | ↑ LDH | ↔ F2,6BP | ||||||||
| -Cisplatin resistant human ovarian carcinoma (A2780/DDP) | ↑ HK2 | ↑ | ↑ glucose uptake | ↓ | ↓ | ↓ citrate | -Cisplatin resistant cells had higher glycolysis than cisplatin sensitive cells. | [53] | ||
| -Cisplatin sensitive human ovarian carcinoma (A2780) (control) | ↑ LDH | |||||||||
The arrow ↑ or ↓ denotes a significant increase or decrease in the metabolic-related finding with a P value <0.05. The arrow ↑↑ or ↓↓ denotes a significant increase or decrease in the metabolic-related finding with a P value <0.01. Abbreviations: BEC index; BioEnergetic Cellular index, CoxVb; cytochrome c oxidase subunit Vb, ECAR; extracellular acidification rate, F2,6BP; fructose-2,6-bisphosphate, GAPDH; glyceraldehyde 3-phosphate dehydrogenase, HGSC; high-grade serous adenocarcinoma, HK2; Hexokinase 2, LDH; lactate dehydrogenase, OCR; oxygen consumption rate, OXPHOS; oxidative phosphorylation, PDK; pyruvate dehydrogenase kinase, PFK; Phosphofructokinase, PGC-1α; peroxisome proliferator-activated receptor gamma coactivator 1-alpha, ROS; Reactive oxygen species.