Figure 3.
Fra inhibits OGD/R-induced H9C2 cardiomyocyte ferroptosis by upregulating HO-1. H9C2 cells were cultured in DMEM medium containing 100 μM Fra for 24 h and then subjected to OGD/R. A and B. qRT-PCR and WB analysis of HO-1 mRNA and protein expression in H9C2 cells treated with OGD/R alone or in combination with Fra. C and D. qRT-PCR and WB analysis of HO-1 mRNA and protein expression in H9C2 cells transfected with si-ctrl or si-HO-1. E and F. qRT-PCR and WB analysis of HO-1 mRNA and protein expression in H9C2 cells transfected with pcDNA-HO-1 or pcDNA-ctrl. G. MTT assay was performed to determine the cell viability. H. LDH detection kit was applied to evaluate the activity of LDH. I-K. The levels of Fe2+, MDA and GSH were detected using commercial kits. L and M. qRT-PCR and WB analysis of GPX4 and HO-1 mRNA and protein expression in H9C2 cells. *P<0.05 vs. si-ctrl group or pcDNA-ctrl group; #P<0.05 vs. OGD/R group; $P<0.05 vs. Fra + OGD/R group.