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Fra inhibits OGD/R-induced H9C2 cardiomyocyte ferroptosis via AKT/Nrf2/HO-1 pathway. (A) MTT assay was performed to determine the cell viability. (B) LDH detection kits were applied to evaluate the activity of LDH. (C-E) The levels of Fe2+, MDA and GSH were detected using commercial kits. (F) qRT-PCR and (G) WB analysis of GPX4 mRNA and protein expression in H9C2 cells. #P<0.05 vs. OGD/R group; $P<0.05 vs. Fra + OGD/R group.
