Figure 5.

CAFs-derived exosomes are involved in the enhanced expression of LncRNA SNHG17 in osteosarcoma. (A) Western blot analysis of fibroblast-related markers (fibronectin, a-SMA) in the NFs and CAFs. (B) The expression levels of LncRNA SNHG17 in normal fibroblasts and cancer-associated fibroblasts isolated from OS patients were detected by qRT-PCR, n = 5. (C) The cell viability of SJSA-1 and HOS cells were determined after different condition medium treatments. (D) The colony assay and (E) migration assay of SJSA-1 and HOS cells after different treatments were performed. Bar = 20 μm. *P<0.05, **P<0.01, ***P<0.001 vs. the sh-NC group. #P<0.05, ##P<0.01, ###P<0.001 vs. the CAF-medium group.