Figure 7.

CAFs-derived exosomes transfer SNHG17 promotes the progression of osteosarcoma via inhibiting MMP2. (A) The expression of NOTCH signaling, Wnt pathway, NF-κB signaling, and Hedgehog signaling related genes in SJSA-1 and HOS cells after SNHG17 depletion were performed by qRT-PCR analysis. (B) Representative blots and statistical analysis for MMP2 in SJSA-1 and HOS cells after different treatments were performed. (C) Cells were treated by NFs-exosomes, CAFs-exosome, or CAFs-exosome (MMP2 KD), and the cell viability was determined by CCK-8 assay. (D) The colony assay and (E) migration assay of SJSA-1 and HOS cells after different treatments were performed. Bar = 20 μm. (F) Spearman correlation analysis determined the relationship between the levels of SNHG17 and MMP2 in OS cancer tissues from TCGA database. *P<0.05, **P<0.01, ***P<0.001 vs. the sh-NC group. #p<0.05, ##P<0.01, ###P<0.001 vs. the CAFs-exosome group.