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. 2021 Sep 15;13(9):10094–10111.

Figure 8.

Figure 8

SNHG17 activates the MMP2 by acting as a competing endogenous RNA sponge for miR-2861. A. The miRNAs targeting MMP2 and SNHG17 were predicted by four online databases. The overlapping miRNAs were performed by the Venn diagram. B. The expression level of miR-2861 and miR-4700-3p in normal tissues and tumor tissues were analyzed by qRT-PCR. C. The binding site between SNHG17 and miR-2861, or the binding sites between MMP2 and miR-2861, was predicted by RNAhybrid databases. D. Luciferase activity in HEK293T cells co-transfected with miR-2861 mimic, inhibitor or related controls (mimic-NC, inhibitor-NC), and a vector containing SNHG17 WT or SNHG17 MUT 3’-UTR, or vectors containing MMP2 WT or MMP2 MUT 3’-UTR. E. Immunoprecipitation using anti-AgO2 antibody or IgG followed by Western blot analysis. Immunoprecipitated RNA was isolated by TRIzol reagent, and the level of miR-2861 or SNHG17 was analyzed by qRT-PCR. ***P<0.001 vs. the IgG group. F and G. The mRNA and protein expression level of MMP2 in HOS cells after different transfections were determined by western blot or qRT-PCR. *P<0.05, **P<0.01, ***P<0.001.