The overexpression of miR-34b-5p can inhibit the proliferation of HNCs and induce apoptosis, and the effects cab be partially reversed by CALB1. A: Measuring the cell viability using CCK8; B: Measuring the apoptosis using Annexin V-FITC/PI double labeling; C: Measuring the protein expressions of CALB1, TH, and Bcl-2 using Western blot. Compared with the NC group, *P<0.05; compared with the miR-34b-5p mimic group, ^P<0.05. N=18, and the experiment was repeated 3 times. HNCs: hippocampal neuronal cells.