Figure 4.

miR-1913 inhibited the cell viability, proliferation, and cell metastasis via targeting NRSN2. A. The NRSN2 expression was determined using western blot. B. The cell viability was determined using CCK8 assays in the 143B and U2OS cells at 24 h, 48 h and 72 h. C. The cell proliferation was determined using colony formation assays with the 143B and U2OS cells co-transfected with NRSN2 siRNA and the miR-1913 inhibitor. D, E. The cell migration and invasion were determined using wound healing and transwell assays in the 143B and U2OS cells co-transfected with NRSN2 siRNA and the miR-1913 inhibitor. Scale bar: 100 µM, 200×. F. The protein N-cadherin and E-cadherin expressions were measured using western blot in the 143B and U2OS cells co-transfected with NRSN2 siRNA and the miR-1913 inhibitor. **P<0.01, ***P<0.001.