FIG. 7.

Neurite outgrowth in N1E-115 cells does not require the JNK or ERK pathway. (a) Transfection of SEK-AL (A) or MEK-AL (E) dominant negative constructs in serum-starved conditions and cotransfections as indicated. At 20 h after transfection, cells were fixed and stained. (b) Activation of JNK in N1E-115 cells following transfection with expression vectors. Following transfection, cell lysates were prepared, and equal quantities of proteins were electrophoresed through polyacrylamide gels, Western immunoblotted, and probed with anti-phospho-JNK antibodies.