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. 2021 Oct 11;19:312. doi: 10.1186/s12951-021-01045-6

Fig. 5.

Fig. 5

Comparison of droplet generation, emulsion, and library preparation and sequencing on the microfluidics-based scRNA-seq methods. A inDrop lyses single cells and then barcodes their mRNA with barcoded hydrogel microspheres in droplets; B Drop-seq applies barcoded beads capturing single cell mRNA and then released from the drops and performed reverse transcription; C 10× Genomics uses Gel bead in EMulsion (GEM) for encapsulating thousands of single cells, and then immediately lysis cells for reverse transcription