Figure 5.

LncRNA RP11-147L13.8 was specifically interacted with the bZIP domain of c-Jun protein

(A) The structure of lncRNA RP11-147L13.8. (B) Immunoblotting detection of c-Jun protein in the pull-down samples is shown. The full-length sense biotinylated-RP11-147L13.8 and truncated biotinylated-RP11-147L13.8 sequence (no. 1 deletes 52–325 bp; no. 2 deletes 1–462 bp; no. 3 deletes 2,415–2,875 bp; no. 4 deletes 2,215–2,919 bp; no. 5 deletes ∼1,421–2,919 bp) were analyzed. β-actin and c-Jun serve as input control. (C) The RIP and qPCR results determined the enrichment of RP11-147L13.8 binding with each c-Jun domain. (D) The pull-down assay and western blot results for different domains of c-Jun protein in NOZ cell lines, which confirmed that lncRNA RP11-147L13.8 specifically interacted with the bZIP domain.