Figure 1.

SUV39H2 is increased in a cardiomyocyte senescence model induced by H₂O₂. (A–C) Effects of different concentrations of H₂O₂ on senescence of H9C2 cells. (A) SA-β-Gal staining of H9C2 cells treated with H₂O₂ concentration in 0, 25, 50 uM groups. (B) Expression of p21, p53, and SUV39H2 in the cells treated with a concentration gradient was detected by western blotting. (C) RT-PCR was used to quantify the expression of p21, p53, and SUV39H2 in above groups. All the experiments have been repeated independently at least 3 times. Scale bars, 200 μm. ^*P < 0.05, ^**P < 0.01, ^***P < 0.005 when two groups were compared as indicated, or compared to the corresponding control.