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. 2021 Sep 27;13(18):22502–22515. doi: 10.18632/aging.203561

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Copyright: © 2021 Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Melatonin induces apoptosis in gallbladder cancer cells. (A) Apoptosis of GBC-SD and NOZ cells treated with 1 mM melatonin was analyzed by flow cytometry. (B) The percentage of apoptotic cells of GBC-SD and NOZ cells was quantified. (C) Expression of Bax, Bcl-2, cytochrome C, cleaved PRRP, and cleaved caspase-3 was investigated by Western blot after GBC-SD cells were treated with 1 mM melatonin. (D) The relative expression of the apoptotic markers was quantified in GBC-SD cells. (E) Expression of Bax, Bcl-2, cytochrome C, cleaved PRRP and cleaved caspase-3 was investigated by Western blot after NOZ cells were treated with 1 mM melatonin. (F) The relative expression of the apoptotic markers was quantified in NOZ cells. Three biological replicates were performed. Data are presented as mean ± SD. Mel, melatonin; ^***P < 0.001; ^**P < 0.01; ^*P < 0.05; ns, no significance.