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. 2021 Sep 29;13(18):22556–22570. doi: 10.18632/aging.203569

Figure 2.

Figure 2

The effect of Val pre-treatment and DPI followed by DOX-exposure on expression of proteins and ROS level in H9c2 cells. (A) Western blot analysis of Val and DOX-treatment on AT1R, NOX2, NOX4, caspase-3, cleaved caspase-9 protein levels. (B) Densitometry analysis of the protein bands of AT1R, NOX2, NOX4, caspase-3, cleaved caspase-9 proteins. (C) Western blot analysis of DPI and DOX-treatment on caspase-3, cleaved caspase-9 protein levels. (D) Densitometry analysis of the protein bands of caspase-3, cleaved caspase-9 proteins. (E) Effect of DPI and DOX on ROS levels at 12 h time point. (F) Microscopic analysis of DPI and DOX-treatment on ROS levels by DCF Fluorescence. *P<0.05 vs. Control; †P<0.05 vs. Dox. Dox, doxorubicin; Val, valsartan.