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. 2021 Sep 23;13(19):4745. doi: 10.3390/cancers13194745

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Differentially expressed miRNAs (DEmiRs) identified in the comparison between tumoral tissues (TT) and non-neoplastic tissues (NNT) by microarray analysis (n = 11) and chosen for validation in the independent cohort of penile cancer (PeC) patients (n = 13). (A) Hierarchical clustering analysis shows that the nine DEmiRs chosen for validation were able to cluster samples according to group. The reason for the selection of each DEmiR is indicated in parenthesis (FC: fold change; BR: Biological Relevance; SCC and PeC: previously described at Squamous Cell Carcinoma and penile cancer, respectively); (B) The box-plots represent the comparison of the expression levels of the nine selected DEmiRs in the TT (n = 13) relative to NNT (n = 13) in the independent validation set of samples; only miR-149-5p was not validated; * p < 0.05.