Figure 6.

BLI and OE-MSOT responses to KGP265 were strongly correlated in different tumor types. (A) The tumor oxygen supply capacity is determined by the amplitude of the vascular response (ΔsO₂) to the oxygen gas breathing challenge. A change in the ΔsO₂ (ΔΔsO₂) is indicative of vascular development or impairment and is shown for each tumor type with respect to the interventions at 2 and 24 h: RENCA tumors: saline control (yellow, n = 3) and KGP265 (grey n = 3) and 4T1 tumors: saline control (orange, n = 3) and KGP265 (blue, n = 4). Individual data points represent the mean ± SD. Fisher’s PLSD analysis of variance (ANOVA) indicated that 4T1 and RENCA tumors both showed a significantly reduced vascular response to the oxygen gas breathing challenge within 2 h (p < 0.0001) of the administration of KGP265, which remained depressed at 24 h (p < 0.001). ***, p < 0.001. (B) The corresponding relative BLI intensity changes observed for these tumors. Individual data points represent the mean ± SD. ANOVA based on Fisher’s PLSD indicated that the 4T1 and RENCA tumors both showed significantly reduced light emission within 2 h (p < 0.001), which remained significantly depressed at 24 h after the administration of KGP265 (p < 0.001).