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. 2021 Oct 5;13(19):4994. doi: 10.3390/cancers13194994

Figure 2.

Figure 2

The miR-27a/FOXJ3 axis affects mitochondrial biogenesis. (A) Immunoblot and (B) qRT-PCR analysis of PGC1-α, NRF1, and TFAM as mitochondrial biogenesis markers in miR-27a_KD and miR-27a_OE cells with respect to their relative Scr_KD and Scr_OE controls in basal conditions. (C) Assessment of the same markers as in (A) after the transfection of the TSBs or the Neg_CTR. The results shown in (A) and (C) are representative of at least two performed experiments, normalized to the mean ± SEM, and expressed as protein levels with respect to α-tubulin as a loading control. The α-tubulin shown is from a representative experiment. Statistical significance was considered when * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, or **** p ≤ 0.0001 (t-test); ns= not significant. File S1: Original Western blots.