Figure 6.

FOXJ3 is a target of miR-27a in a mouse xenograft model in vivo and in a CRC dataset. (A) Representative immunoblot for FOXJ3, NRF1, SDHB, and COX II on extracts from xenografts of HCT116 and HT29 cells in immune-compromised mice that were intratumorally injected with an miR-27a inhibitor or mimic, respectively, or scrambled controls. The data shown are the mean of experiments ± SEM performed on extracts from N = 3 mice per each type, expressed as protein levels with respect to β-actin as a loading control. Statistical significance was considered when * p ≤ 0.05, ** p ≤ 0.01, or *** p ≤ 0.001 (t-test). (B) Correlation of FOXJ3 with miR-27a expression in TCGA-COADREAD patients; N = 548 and p = 0.0004. (C) The bar graph illustrates the top Gene Ontology biological processes, identified via Metascape, using a discrete color scale to represent statistical significance (a deeper color indicates a smaller p-value). (D) This drawing depicts the key factors involved in mitochondrial biogenesis and dynamics, their reciprocal modulation, and their overall regulation by miR-27a. File S1: Original Western blots.