FIG. 4.

Protein blot analysis of different RSP5 alleles. Extracts from two rsp5-25, rsp5-Y647och, rsp5-W650L, and RSP5 strains were prepared, and the total amount of Rsp5 protein was detected by protein blot analysis by using Rsp5 antibody. The full-length Rsp5 proteins (92 kDa) is indicated as Rsp5. In rsp5-25 and rsp5-Y647och strains, an additional truncated form of Rsp5 (72 kDa) was observed and is indicated as Rsp5*. This is due to the ochre codon present at the position of residue 647. The SUP4-o suppressor also present in these strains recognizes the ochre codon to produce full-length Rsp5. However, since SUP4-o suppression is only partial, both full-length and truncated forms of Rsp5 are observed. The other bands are background obtained with the Rsp5 antibody.