TABLE 1.
Strains used in this study
| Straina | Genotype | Source |
|---|---|---|
| W1588-4A | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 | Rothstein lab |
| W1100-3B | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 SUP4-o::CAN1-URA3::sup4+ | Rothstein lab |
| W1088-1A | MATα ade2-1 can1-100 his5Δ leu2-3,112 trp1-1 ura3-1 | Rothstein lab |
| W1088-10D | MATα ade2-1 can1-100 his5Δ leu2-3,112 trp1-1 ura3-1 | Rothstein lab |
| W1698-11C | MATα ade2-1 can1-100,x his3-11,15 leu2-3, 112 trp1-1 ura3-1 RAD5 rad1::HIS5 rad52::HIS5 rfa1-D228Y SUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| J709 | MATα ade2-1 can1-100,x his3-11,15 leu2-3, 112 trp1-1 ura3-1 RAD5 rad1::HIS5 rad52::HIS5 rfa1-D228Y rsp5-25 SUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| J714 | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rad1::HIS5 rad52::HIS5 rfa1-D228Y rsp5-26 SUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| W1713-1B | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rsp5-25 SUP4-o::CAN1-HIS3::sup4+ | This study |
| W1713-22D | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rad1::HIS5 rad52::HIS5 rfa1-D228Y rsp5-25 SUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| U1025 | MATa/α ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rsp5Δ SUP4-o::CAN1-HIS3::sup4+ | This study |
| W1713-1A | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rfa1-D228Y SUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| W2013-4B | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rsp5-Y647och SUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| W2012-2D | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rsp5-W650L SUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| W1713-22A | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rfa1-D228Y rsp5-25 SUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| W2013-11C | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rfa1-D228Y rsp5-Y647ochSUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| W2012-15C | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rfa1-D228Y rsp5-W650L SUP4-o::CAN1-HIS3::sup4+ leu2ΔEcoRI::URA3::leu2BstEII | This study |
| W2015-1A | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rfa1Δ rsp5-25 SUP4-o::CAN1-HIS3::sup4+ pWJ709-GAL1-rfa1-D228Y | This study |
| W2015-4A | MATα ade2-1 can1-100,x his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 rfa1Δ SUP4-o::CAN1-HIS3::sup4+ pWJ709-GAL1-rfa1-D228Y | This study |
| W2016-1B | MATα ade2-1 can1-100,x his3-11, 15 leu2-3, 112 trp1-1 ura3-1 RAD5 rfa1Δ SUP4-o::CAN1-HIS3::sup4+ pWJ708-GAL1-RFA1 | This study |
| CMY763 | MATα cim3-1 ura3-52 leu2Δ1 | Carl Mann |
| CMY765 | MATα cim5-1 ura3-52 leu2Δ1 his3Δ-200 | Carl Mann |
| CMY826 | MATa ura3-52 leu2Δ1 his3Δ-200 trp1Δ63 lys2-801 ade2-101 bar1::HIS3 | Carl Mann |
a
All strains used are derivatives of W303-1B (55). The genotypes are identical to that of W303-1B except where noted. In most instances several strains with the same genotype (except the mating type) were used. However, only one strain is noted. In addition, can1-100,x is an allele of can1-100 in which an unidentified secondary mutation prevents suppression by SUP4-o.