Figure 1.

Experimental design. PBMCs were isolated from Buffy Coats and monocytes enriched. Afterward, these were differentiated to macrophages and stimulated with 100 ng/mL LPS or the AhR-ligands BaP (2 µM), I3AA (50 µM) and IAld (50 µM) with or without additional LPS for 2, 4, 16 and 24 h. Cell lysates were used to perform global proteomics and subsequent data analysis.