CD147 regulates LECs lymphangiogenic markers through PROX-1 transcription factor. LECs were transfected with scrambled or PROX-1 siRNA and treated or not with rhCD147. (a) qRT-PCR of PROX1, PDPN, and LYVE1 expression using PPIA as a reference. Columns indicate means of three independent experiments carried out in triplicate; bars indicate SD; * p < 0.05, *** p < 0.0001 for siRNA-PROX-1 vs. siRNA-scrambled. ns, nonsignificant, # p < 0.05, ### p < 0.0001 for treated vs. untreated cells. (b) Representative confocal images of immunofluorescence staining for PROX-1, Podoplanin and LYVE-1 (63× magnification).