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H-NS inhibits Congo red binding. (A and C) UPAB1 and derivatives strain spotted in YESCA Congo red agar plates. (B and D) Quantification of Congo red binding (excitation wavelength at 485 nm and the emission at 612 nm). The values represent the means and standard deviations from five (B) and four (D) independent experiments. A t test was performed by comparison to the wild type (**, P ≤ 0.003; *, P ≤ 0.03; ns, not significant).
