FIG 7.

CsgG and PNAG are involved in extracellular matrix production. SEM analysis of UPAB1p-, p-ΔcsgG, p-ΔcsgG/c, and p-ΔpgaA-D (as a control) was performed. Cells were grown in YESCA media with 4% DMSO in 24-well plates with glass coverslips. After overnight growth, the glass coverslips were removed, washed (150 mM cacodylate buffer with 2 mM CaCl₂), fixed (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.2% tannic acid in 150 mM cacodylate buffer [pH 7.4] with 2 mM CaCl₂), and treated for observation. The bottom panel is a magnification of the white square in the top panel. The scale bars indicate 1 μm for the top panel and 200 nm for the bottom panel.