FIG 4.
TssAVF, TssM, and TasR are all required to restore killing activity in mutants. (A) Fluorescence microscopy images of ES114 at 24 h following coincubation with transposon mutants carrying the empty vector or expression vectors for tssAVF, tssM, tasR, tssAVF-tssM, tssM-tasR, or tssAVF-tssM-tasR. All images were taken after a 24 h incubation on LBS agar supplemented with 0.1 mM IPTG. The scale bar is 2 mm. Assays were performed at least three times (n = 3) and a representative experiment is shown.