FIG 5.
Overexpression of TssAVF or TssM prevents target killing. (A) Total CFU counts of the target strain (ES114) at 5 h and fluorescence microscopy images of ES114 at 24 h following coincubation with wild-type ES401 carrying expression vectors for tssFG, tssAVF, tssM, or tasR on LBS agar supplemented with either 0.01, 0.1, 0.5, or 1.0 mM IPTG as indicated. The dashed line indicates the average ES114 CFU at 0 h. (B) Percentage of wild-type ES401 CFU recovered after 5 h following an incubation on LBS agar supplemented with kanamycin and 1.0 mM IPTG to induce expression of the indicated genes. The dashed line indicates 100% recovery at 5 h, where >100% indicates net cell growth and <100% indicates net cell death. All assays were performed at least three times (n = 3), and a representative experiment is shown. All scale bars are 2 mm.