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SpoVAD and SpoVAE interact in sporulating cultures. C. difficile cells transformed with the indicated plasmids were grown on sporulation medium. Luminescence of the sporulating culture was assayed after 2 days of growth. Averages from 4 biological replicates are shown. **, P < 0.001 as determined by one-way ANOVA using Dunnett’s multiple-comparison test.
