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. 2021 Oct 1;13(19):4957. doi: 10.3390/cancers13194957

Figure 5.

Figure 5

NCL targeting by N6L increases 5′TOP mRNA translation by activating mTOR pathways. (A) mTOR pathway activation was analyzed using Western blotting in mPDAC cells, non-treated (NT) or treated with N6L at 50 µM for 48 h (lanes 1,2) or after NCL siRNA depletion (lanes 3,4). γ and β represent hyper- and α hypo-phosphorylated forms of 4EBP1. The phosphorylated-to-total protein ratio was quantified for RPS6, AKT, and 4EBP1. (B,C)—mTOR pathway activation was analyzed in mPDAC, non-treated (NT), or treated (T) with N6L at increasing doses using Western blotting (B) and immunofluorescence staining (C) by anti-phosphorylated RPS6 (red) and DAPI for nuclei (blue). (D)—Western-blotting analysis of LARP1, RPS6, 4EBP1, and EiF4E expression in mPDAC treated or not with N6L at 30 and 50 µM for 48 h.