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. 2021 Sep 23;22(19):10212. doi: 10.3390/ijms221910212

Figure 4.

Figure 4

Increased IL-1β mRNA, protein levels, and distribution in the skeletal muscle of HFD-fed mice. (a) Relative IL-1β mRNA levels in isolated adult FDB fibers from NCD- and HFD-fed mice measured by RT-qPCR and normalized to GAPDH. Values represent the mean ± SEM from six independent determinations. ** p < 0.01, determined by Student’s t-test statistical analysis. (b) Representative Western blot showing IL-1β protein content in homogenates prepared from the FDB muscle isolated from NCD- or HFD-fed mice at 8 weeks. Each lane was loaded with 60 μg of total protein. Values represent the mean ± SEM (n = 7). ** p < 0.01, determined by Student’s t-test. (c) Representative confocal images of isolated adult FDB fibers from NCD- and HFD-fed mice, illustrating the localization of IL-1β (green) and NLRP3 (red). DAPI staining of the nuclei is shown in blue. The area under the curve (AUC) was measured on white squares, and represented as fluorescence intensity. Scale bar: 20 μm.