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. 2021 Sep 23;22(19):10212. doi: 10.3390/ijms221910212

Figure 7.

Figure 7

MCC950 improves the insulin-mediated GLUT4myc-eGFP translocation to the cell surface. (a) Representative images showing GLUT4 distribution in fibers electroporated with the GLUT4myc-eGFP (green) chimera plasmids. The fibers were isolated from NCD- or HFD-fed mice. The extracellular myc epitope (shown in red) was detected by immunofluorescence in non-permeabilized fibers treated with 100 nM insulin (20 min), 10 μM MCC950 (overnight), or both. Scale bar: 30 μm. (b) The bar graph shows the quantification of the effects presented in (a). Values represent the mean ± SEM (n = 4–5 independent experiments). Statistical analysis was performed by the Kruskal–Wallis test followed by Dunn’s post-hoc test. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the basal NCD condition, and # p < 0.05 while ## p < 0.01 vs. the basal HFD condition.