Figure 3.

Effects of plasma exosomes-derived from OSA patients before CPAP treatment (OSA-V1) and after adherent CPAP treatment for 1 year (OSA-V2) on melanoma cell migration. Cells were seeded in 24-well trans-well inserts with 8 μm pore size. Cells were allowed to migrate for 40 h, and then were stained with 0.2% crystal violet solution followed by phase contrast imaging. The migration rate was determined by counting the number of cells contained in the photos of 5 different fields at original magnification. (A) represents average of different fields for each subject for each group using CRL-1424 cells, (B) CRL-1629, and (C) CRL-1675, (n = 20/group), with statistical significance set at p < 0.05.