Figure 7.

Forced expression of ATXN1 gene reduces sorafenib resistance and MET in PLC/PRF5-R2 and HLF cells. (a,b) PLC/PRF5-R2 and HLF cells were transfected with ATXN1 expressing vector (PLC/PRF5-R2-ATXN1, HLF-ATXN1) or the control vector (PLC/PRF5-R2-Vector NC, HLF-Vector NC). They were exposed to sorafenib, and cell viability was assessed by WST assay. (c,d) Expression of ATXN1 and EMT markers (E-cadherin, Snail, vimentin) were examined by western blot analysis. Densitometry analysis indicates relative protein levels from one representative of three independent experiments. Numbers below the bands represent protein expression normalized to β-actin.