Figure 6.

Overexpression of miR-33b inhibits tumor dissemination of metastatic ovarian cancer cells in vivo. (A–D) miR-33b overexpressing or scrambled control ES-2 cells with GFP label were intraperitoneally injected into 6–8 weeks old female SCID mice in a group of 5. The bodyweight of the mice was continuously monitored. The mice were sacrificed about 3 weeks later, and the body weight, tumor mass, number of tumor nodules, and ascites volume were measured. OE: overexpression. (E) GFP-labelled ES-2 cells were disseminated in the abdominal cavity, and tumor nodules were formed, which were visualized by fluorescence stereomicroscopy. BF: bright field. (F) The expression of TAK1, FASN, CPT1A, P-IKKα/β, P-IκBα, Ki67 in tumor tissues of miR-33b overexpression and scrambled control groups were measured by IHC. scale bar: 100 μm.