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. 2021 Oct 2;13(19):4966. doi: 10.3390/cancers13194966

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cytometry-based isolation following CD44v6 staining efficiently discriminated tumor cells from hematopoietic cells. (A) Flowchart summarizing the strategy used to purify CTC in patient blood samples through fluorescent-activating cell sorting (FACS). Detailed protocol is described in the Materials and Methods section. (B). Photos of GFP-positive CTCs cultured after cell sorting following staining with an antibody directed against CD44v6, observed with a microscope (fluorescent for the left photo and bright field for the middle one; the right photo is merged). Scale bars represent 50 µm.

Cytometry-based isolation following CD44v6 staining efficiently discriminated tumor cells from hematopoietic cells. (A) Flowchart summarizing the strategy used to purify CTC in patient blood samples through fluorescent-activating cell sorting (FACS). Detailed protocol is described in the Materials and Methods section. (B). Photos of GFP-positive CTCs cultured after cell sorting following staining with an antibody directed against CD44v6, observed with a microscope (fluorescent for the left photo and bright field for the middle one; the right photo is merged). Scale bars represent 50 µm.