Table 3.
The qRT-PCR primer sequences and annealing temperature.
| Gene | Primer Sequences (5′→3′) | TM (°C) |
|---|---|---|
| β-actin | F: GTGACGTTGACATCCGTAAAGA | 60 |
| R: GCCGGACTCATCGTACTCC | ||
| U6 | F: CTCGCTTCGGCAGCACA | 60 |
| R: AACGCTTCACGAATTTGCGT | ||
| C/EBPα | F: GCGGGAACGCAACAACATC | 62 |
| R: GTCACTGGTCAACTCCAGCAC | ||
| CDK4 | F: GTCAGTTTCTAAGCGGCCTG | 60 |
| R: CACGGGTGTTGCGTATGTAG | ||
| FABP4 | F: AAGGTGAAGAGCATCATAACCCT | 52 |
| R: TCACGCCTTTCATAACACATTCC | ||
| SCD-1 | F: TTCTTGCGATACACTCTGGTGC | 63.3 |
| R: CGGGATTGAATGTTCTTGTCGT | ||
| ACADL | F: TGCCCTATATTGCGAATTACGG | 63.3 |
| R: CTATGGCACCGATACACTTGC | ||
| ACOX2 | F: CATCCAACGTGACCCAGTGTT | 63.3 |
| R: AAATGCGTTCAGGACCGTCTT | ||
| CDK2 | F: GCGACCTCCTCCCAATATCG | 60 |
| R: GTCTGATCTCTTTCCCCAACTCT | ||
| LPL | F: GGTTGCGCGTAGAGAGGATG | 59.6 |
| R: CTCACGCTCTGACATGCCTTC | ||
| PPARα | F: TACTGCCGTTTTCACAAGTGC | 62 |
| R: AGGTCGTGTTCACAGGTAAGA | ||
| Adipor2 | F: TGTTCCTCTTAATCCTGCCCA | 52.8 |
| R: CCAACCTGCACAAGTTCCCTT | ||
| FAS | F: AGGTGGACTGGATACACAGAC | 62 |
| R: TCTCCTGCCCAAACTCTTTGC | ||
| Cyclin E1 | F: CTCCGACCTTTCAGTCCGC | 60 |
| R: CACAGTCTTGTCAATCTTGGCA | ||
| p21 | F: GATGGCTTCGACACCATTCC | 60 |
| R: AGACGACACAGGTGAGGAAG | ||
| Cyclin D1 | F: CTCCGTATCTTACTTCAAGTGCG | 60 |
| R: CTTCTCGGCAGTCAAGGGAA | ||
| p53 | F: CTCTCCCCCGCAAAAGAAAAA | 60 |
| R: CGGAACATCTCGAAGCGTTTA | ||
| mmu-miR-126b-5p | ATTATTACTCACGGTACGAGTT | 60 |