Figure 2.

Roots of TdPP1 expressing lines are hyposensitive to exogenous eBL. (a–c) Effect of exogenously applied eBL on meristem size. Confocal images of 5-day-old WT, TdPP1-GFP OE lines and bes1-D grown under control conditions (MS; a), or treated with 0.4 nM (b) or 1 nM eBL (c). Seedlings were treated with ClearSee, stained with calcofluor-white. Arrows indicate boundary between the proximal meristem and the elongation zone of the root. Scale bars: 100 µm. (d) Meristem size of WT, TdPP1-GFP transgenics and bes1-D exposed or not to eBL. Meristematic zone size was defined as the region of isodiametric cells from the QC up to the cell that was twice the length of the immediately preceding cell. Letters indicate statistically significant differences between WT vs TdPP1-GFP OE (L7, L10 and L13) and bes1-D as determined by one-way ANOVA followed by Tukey’s multiple comparison test (p-value < 0.05). Data represent mean values of 15 measurements and error bars are SD. (e) Size of elongation zones of WT, TdPP1-GFP transgenics and bes1-D exposed or not to eBL. Elongation zone was considered as the region going from the end of the meristematic zone to the beginning of differentiation zone (emergence of first root hair). Letters indicate statistically significant differences between WT vs TdPP1-GFP OE (L7, L10 and L13) and bes1-D as determined by one-way ANOVA followed by Tukey’s multiple comparison test (p-value < 0.05). Data represent mean values of 15 measurements and error bars are SD.