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. 2021 Sep 27;22(19):10424. doi: 10.3390/ijms221910424

Figure 3.

Figure 3

Relative expression of BR-biosynthesis genes CPD and DWF4. (a) Analysis of CPD and (b) DWF4, BR-responsive gene expression in Arabidopsis WT plants, TdPP1-GFP OE lines (L7, L10 and L13) and bes1-D. Plants were grown on half-strength MS medium for 7 days and transferred to half MS-strength liquid medium without or with 100 nM BL for 1 h. Seedlings were collected and the expression level of CPD and DWF4 was quantified by qRT-PCR using the ACT2 and GAPC2 gene as reference genes. The expression of CPD and DWF4 was normalized to the expression of ACT2. Data represent mean values and error bars are SD from the average of three biological replicates (10 seedlings per biological replicate). Letters indicate statistically significant differences between WT vs TdPP1-GFP OE (L7, L10 and L13) and bes1-D as determined by one-way ANOVA followed by Tukey’s multiple comparison test (p-value < 0.05).