Table 1.

Endomembrane-related genes used in this study.

Gene	Identifier	Role and Localisation	References
AtVSR2	AT1G71980.1	Involved in the trafficking of vacuolar proteins. May function as a sorting receptor for protein trafficking to the protein storage vacuole (PSV).	[20]
AtATG8	AT1G62040.2	Ubiquitin-like modifier involved in autophagosome formation. May mediate the delivery of the autophagosomes to the vacuole via the microtubule cytoskeleton; belongs to the ATG8 family	[21]
AtEXO70	AT5G61010.1	Essential component for the formation and the recruitment of exocyst subunits to the exocyst-positive organelle (EXPO), a secreted double membrane structure also called extracellular exosome.	[22]
AtVAMP722	AT2G33120.2	Involved in the targeting and/or fusion of transport vesicles to their target membrane. Associated with biotic stress response. Defines a complex with SYP121.	[23,24]
AtRMR1	AT5G66160.1	Involved in the trafficking of vacuolar proteins. Functions probably as a sorting receptor for protein trafficking to the protein storage vacuole (PSV) by binding the C-terminal vacuolar sorting determinant (VSD) of vacuolar-sorted proteins	[25]
AtVTI11	AT5G39510.1	May function as a v-SNARE responsible for targeting AtELP-containing vesicles from the trans-Golgi network (TGN) to the prevacuolar compartment (PVC). Promotes the formation of vacuolar membrane ‘bulbs’.	[26]
AtSYP121	AT3G11820.1	Encodes a syntaxin localized at the plasma membrane. Functions in positioning anchoring of the KAT1 K+ channel protein at the plasma membrane. Additionally, functions in non-host resistance against barley powdery mildew. Forms a complex with VTI11 and SYP51.	[10,27]
AtSYP23	AT4G17730.2	Cytosolic syntaxin with no transmembrane domain. May function in the docking or fusion of transport vesicles with the prevacuolar membrane.	[28]
AtSYP51	AT1G16240.1	Involved in the transport of chitinase to the PSV. Interacts with VTI12.	[29]
AtSYP52	AT1G79590.2	Involved in the route to the Lytic vacuole. Forms a complex with VTI11.	[29]
AtSYP61	AT1G28490.1	Vesicle trafficking protein that functions in the secretory pathway. Involved in osmotic stress tolerance and in abscisic acid (ABA) regulation of stomatal responses. Forms a complex with VTI12.	[10]
AtSYP22	AT5G46860.1	Syntaxin-related protein required for vacuolar assembly. Localized in the vacuolar membranes.	[28]
AtVAMP723	AT2G33110.1	Localized at the endoplasmic reticulum. Involved in the targeting and/or fusion of transport vesicles to their target membrane.	[30]
AtVPS45	AT1G77140.1	A peripheral membrane protein that associates with microsomal membranes, likely to function in the transport of proteins to the vacuole. Has been described as a positive regulator for PSV route. Forms a complex with VTI12 and SYP61.	[31]
AtVSR1	AT3G52850.1	Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles. Required for the sorting of 12S globulin, 2S albumin and maybe other seed storage proteins to protein storage vacuoles (PSVs) in seeds.	[32]
AtVTI12	AT1G26670.1	Normally localizes to the trans-golgi network and plasma membrane. Involved in protein trafficking to protein storage vacuoles	[26,33]
AtVSR2	AT2G14720.1	Vacuolar sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles; belongs to the VSR (BP-80) family.	[34]