Figure 1.

HeC induces fibroblast migration, but not myofibroblast transformation or inflammation; (A) Migration of CTRL and HeC NRCFs after scratch stimuli with representative images 0, 8 and 24 h after scratch with 5× magnification; (B) qPCR analysis of markers of myofibroblast transformation (α-SMA, Col1α2, FAP and TGF-β) and inflammatory markers (IL1β and IL6) of CTRL and HeC NRCFs. Vimentin was used as a control for fibroblast phenotype; (C) WB representative bands and relative protein expression of α-SMA and Vimentin compared to DDR2 and TGF-β compared to GAPDH in CTRL and HeC NRCF cells; n = 4–7. Results are represented as mean ± SEM; * p < 0.05 vs. CTRL.