Figure 4.

HeC accelerates fibroblast migration without inducing myofibroblast transformation but does not affect fibrosis and angiogenesis via secreted factors. In vitro HeC protocol composed of 4 times one hour 95% helium 5% CO₂ followed by one hour 95% air 5% CO₂ conditions. NRCF cells migrated faster after HeC treatment, but they did not transform to myofibroblasts. mEV concentration tended to decrease in HeC NRCF supernatant. Supernatant of HeC-treated NRCFs did not induce fibrosis or angiogenesis in naïve cells.