Figure 6.

Effect of chamomile extracts and pure flavones on ROS basal levels. Caco2 cells were grown for 24 h in the absence (ctrl) or in the presence of 100 µg/mL of each extract or 0.5 µg/mL apigenin (Api), 5 µg/mL apigenin-7-O-glucoside (Api 7-G), 1.5 µg/mL luteolin (Lut), or 3 µg/mL luteolin-7-O-glucoside (Lut 7-G), and then incubated with 10 μM DCFH-DA for 30 min. Afterwards, ROS levels were quantified by FACS analysis. Data are reported as percentage of untreated cells (ctrl) arbitrarily set to 100%. Results represent the means ± SEM of 3 independent experiments, each performed in triplicate.