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. 2021 Sep 27;22(19):10410. doi: 10.3390/ijms221910410

Figure 2.

Figure 2

Loss of GDAP1L1 reduced IMQ-induced cytokine production and MAPK and NF-κB phosphorylation. (a) q-PCR analysis (n = 3) of GDAP1L1 and (b) immunoblotting analysis of the GDAP1L1 and CCR7 in control (shLuc) or GDAP1L1-deficient THP1 macrophages by IMQ stimulation. (c) Immunoblotting analysis of MAPKs and p65 phosphorylation levels in the control or GDAP1L1-deficient THP1 macrophages by IMQ stimulation for 4 h. (d) ELISA analysis of the indicated cytokines (n = 4−6) in the supernatants of control or GDAP1L1-deficient THP1 macrophages stimulated with IMQ for 24 h. * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared to the control (shLuc). The data are presented as the mean ± SEM and are representative of at least three independent experiments.